Journal: RSC Advances

Article Title: Improvement of near-infrared (NIR) reflectivity and black color tone by doping Zn 2+ into the Ca 2 Mn 0.85 Ti 0.15 O 4 structure

doi: 10.1039/c9ra07849e

Figure Lengend Snippet: UV-Vis-NIR (a), UV-Vis (b), and NIR (c) reflectance spectra of the Ca 2 Mn 0.85− x Ti 0.15 Zn x O 4− x (0 ≤ x ≤ 0.10) samples.

Article Snippet: The optical reflectance spectra were measured with an ultraviolet-visible-near-infrared (UV-Vis-NIR) spectrometer (JASCO, V-770 with an integrating sphere attachment) with barium sulfate for the visible light region and polytetrafluoroethylene (PTFE) for the NIR region as references.

Techniques:

Journal: RSC Advances

Article Title: Improvement of near-infrared (NIR) reflectivity and black color tone by doping Zn 2+ into the Ca 2 Mn 0.85 Ti 0.15 O 4 structure

doi: 10.1039/c9ra07849e

Figure Lengend Snippet: Color coordinate data and total ( R Tot ) and NIR ( R NIR ) solar reflectance of the Ca 2 Mn 0.85− x Ti 0.15 Zn x O 4− x (0 ≤ x ≤ 0.10) pigments

Article Snippet: The optical reflectance spectra were measured with an ultraviolet-visible-near-infrared (UV-Vis-NIR) spectrometer (JASCO, V-770 with an integrating sphere attachment) with barium sulfate for the visible light region and polytetrafluoroethylene (PTFE) for the NIR region as references.

Techniques:

Journal: RSC Advances

Article Title: Improvement of near-infrared (NIR) reflectivity and black color tone by doping Zn 2+ into the Ca 2 Mn 0.85 Ti 0.15 O 4 structure

doi: 10.1039/c9ra07849e

Figure Lengend Snippet: UV-Vis-NIR reflectance spectra of various black pigments.

Article Snippet: The optical reflectance spectra were measured with an ultraviolet-visible-near-infrared (UV-Vis-NIR) spectrometer (JASCO, V-770 with an integrating sphere attachment) with barium sulfate for the visible light region and polytetrafluoroethylene (PTFE) for the NIR region as references.

Techniques:

Journal: RSC Advances

Article Title: Improvement of near-infrared (NIR) reflectivity and black color tone by doping Zn 2+ into the Ca 2 Mn 0.85 Ti 0.15 O 4 structure

doi: 10.1039/c9ra07849e

Figure Lengend Snippet: Color coordinate data and total ( R Tot ) and NIR ( R NIR ) solar reflectance of the black pigments

Article Snippet: The optical reflectance spectra were measured with an ultraviolet-visible-near-infrared (UV-Vis-NIR) spectrometer (JASCO, V-770 with an integrating sphere attachment) with barium sulfate for the visible light region and polytetrafluoroethylene (PTFE) for the NIR region as references.

Techniques:

Journal: RSC Advances

Article Title: Improvement of near-infrared (NIR) reflectivity and black color tone by doping Zn 2+ into the Ca 2 Mn 0.85 Ti 0.15 O 4 structure

doi: 10.1039/c9ra07849e

Figure Lengend Snippet: Color coordinates and total ( R Tot ) and NIR ( R NIR ) solar reflectance of Ca 2 Mn 0.77 Ti 0.15 Zn 0.08 O 3.92 before and after chemical stability test

Article Snippet: The optical reflectance spectra were measured with an ultraviolet-visible-near-infrared (UV-Vis-NIR) spectrometer (JASCO, V-770 with an integrating sphere attachment) with barium sulfate for the visible light region and polytetrafluoroethylene (PTFE) for the NIR region as references.

Techniques:

Journal: Cell

Article Title: SARS-CoV-2 infection triggers profibrotic macrophage responses and lung fibrosis

doi: 10.1016/j.cell.2021.11.033

Figure Lengend Snippet:

Article Snippet: CD304 (BDCA-4)-PE-Vio770, human, AD5-17F6 , Miltenyi , Cat# 130-113-518.

Techniques: Immunohistochemistry, Plasmid Preparation, Recombinant, Staining, Lysis, Protease Inhibitor, Mass Spectrometry, Sequencing, Modification, Bicinchoninic Acid Protein Assay, Enzyme-linked Immunosorbent Assay, Software

Journal: NPJ Vaccines

Article Title: CD4 + T h immunogenicity of the Ascaris spp . secreted products

doi: 10.1038/s41541-020-0171-z

Figure Lengend Snippet: a For generating Ascaris suum ES-specific T cell lines, PBMCs from healthy donors were stimulated with 40 µg/mL ES antigen for 6 h, enriched for CD40-L + cells and expanded for 2 weeks (see Supplementary Fig. ). Expanded ES-reactive T cells were re-stimulated with or without (w/o) ESF or ESM-antigen-primed, CD3-depleted APC and percentages of CD40-L + antigen-reactive T cells among CD4 + cells are indicated above gates. b Percentages of CD40-L + antigen-reactive T cells among A. suum ES-reactive T cell lines re-stimulated with ESF or ESM antigen, or with mismatched ES antigens for n = 3–5 different donors (left: mean with SEM, right: paired t -test). c Model antigenic mixtures derived from Ascaris ES products are different in protein composition. SDS-PAGE of ES male (ESM) and ES female (ESF) mixtures (40 μg of antigen loaded per well). d A mass-spectrometry-based approach used to determine composition of male and female ES products. The emPAI and the ESF vs . ESM ( 16 O/ 18 O) ratios are defined after tryptic digest defining the total abundance and the differences between ESF and ESM. e Volcano plot showing differences in protein composition determined by mass spectrometric analysis. The depicted log 2 -fold intensity difference (ESM vs. ESF) represents the difference in geometric mean values from 18 O labeling. On the y axis the –log ( p -value) for the difference observed is indicated. Blue symbols indicate ESF-enriched and red corresponds to ESM-enriched. The different size of the corresponding symbols indicates the determined emPAI value as shown in the legend.

Article Snippet: The following monoclonal antibodies reactive with human species were used for staining: CD8-VioGreen (BW135/80, cat.: 130-113-726, 1:50), CD4-APC-Vio770 (VIT4, cat.: 130-113-211, 1:50), CD154/CD40L-PE-Vio770 (5C8, cat.: 130-096-793, 1:10), CD20-VioGreen (LT20, cat.: 130-113-379, 1:50), CD14-VioGreen (TÜK4, 130-113-153, 1:50) (all from Miltenyi Biotec, Bergisch Gladbach, Germany); IL-13-FITC (PVM13-1, cat.: 11-7139-42, 1:20 or 85BRD, cat.: 11-7136-42, 1:20), IL-4-PE (8D4-8, cat.: 12-7049-42, 1:20) (all from Thermo Fisher Scientific); TNFa-Pacific Blue (MAb11, cat.: 502920, 1:100), IFNg-PerCp-Cy5.5 (4S.B3, cat.: 502526, 1:20), CD3-APC (Okt3, cat.: 17-0037-42, 1:50) (all from Biolegend, San Diego, CA, US).

Techniques: Derivative Assay, SDS Page, Mass Spectrometry, Labeling

Journal: NPJ Vaccines

Article Title: CD4 + T h immunogenicity of the Ascaris spp . secreted products

doi: 10.1038/s41541-020-0171-z

Figure Lengend Snippet: a ES products are incubated in vitro with recombinant proteins and adequate buffer conditions. Proteolytic activities used degrade most of the components of the reactions except for MHCII proteins which are subsequently pull down using a conformation specific antibody (L243). b MaxQuant is used for their identification and PLAtEAU defines series of nested peptides and retrieve the consensus peptides and corresponding MS1 intensities according to the MaxQuant output. For each peptide a relative abundance value is retrieved based on the MS1 intensity and the total ion current from the run. This approach yields a list of candidate epitopes with relative abundance values and predicted affinities. c Summary of the performance of the experimental determination of candidate antigens for each condition tested. The overlap between the peptide sets (based on predicted binding cores to facilitate comparisons) for each allotype and ES antigen, and the predicted IEDB immunogenicity score is shown as Venn Diagrams (sized according to numbers, the IEDB set consists of 3678 entries and it is cut in this figure but shown in full in Supplementary Fig. ). d Mapping of the identified potential epitopes to their corresponding protein sources using the intensity color code shown in the legend. e Summary of peptides used to evaluate the performance of the reconstituted in vitro system. The peptide sequence is shown in the first column and underlined is shown the binding core predicted for DRB1*07:01. The corresponding protein source with the amino acid positions covered by the peptide are indicated in the second column. Abbreviated uniprot names are provided. Last columns include the relative and total abundance of each protein sources and the predicted binding affinity for DRB1*07:01 and whether any peptide with the same binding core (underlined) is predicted to be immunogenic by IEDBcd4 prediction tool. f Representative dot blot example for a ESF antigen-specific T cell line generated from an healthy DRB1*07-typed volunteer and re-stimulated with either whole ES antigen (40 µg/mL), no antigen (w/o) or a pool of synthetic peptides (25 µg/mL for each peptide) selected from the in vitro reconstituted HLA-DRB1*07:01 experimental data set ( f ). g Summarizes CD40-L frequencies among CD4 + , indicative for peptide recognition by CD4 + T cells, for whole ES antigen, peptide pool and single peptide re-stimulations. Peptide sequences are indicated in Table ( f ). Combined are data from the same healthy, DRB1*07-typed volunteer from n = 3 separate experiments with n = 2 separate re-stimulations (1st peptide set) or n = 1 experiment with n = 3 separate re-stimulations (2nd peptide set). CD40-L frequencies per experiment were corrected for individual background CD40-L expression of w/o antigen/w/o APC controls (mean with SEM). h Representative dot blots of an Ascaris ESF-specific, DRB1*07T cell line analyzed for Ascaris ESF peptide-specific tetramer staining. Left side indicates overall frequency of ESF antigen specific CD4 + cells after expansion compared to control. Right side shows corresponding tetramer staining with DRB1*07:01-Tet- CLIP (control), Tet- RtBP and Tet Ov17 gated on CD4 + T cells after expansion. Italic numbers indicate calculated Tet + frequency relative to proportion of ESF antigen-specific T cells.

Article Snippet: The following monoclonal antibodies reactive with human species were used for staining: CD8-VioGreen (BW135/80, cat.: 130-113-726, 1:50), CD4-APC-Vio770 (VIT4, cat.: 130-113-211, 1:50), CD154/CD40L-PE-Vio770 (5C8, cat.: 130-096-793, 1:10), CD20-VioGreen (LT20, cat.: 130-113-379, 1:50), CD14-VioGreen (TÜK4, 130-113-153, 1:50) (all from Miltenyi Biotec, Bergisch Gladbach, Germany); IL-13-FITC (PVM13-1, cat.: 11-7139-42, 1:20 or 85BRD, cat.: 11-7136-42, 1:20), IL-4-PE (8D4-8, cat.: 12-7049-42, 1:20) (all from Thermo Fisher Scientific); TNFa-Pacific Blue (MAb11, cat.: 502920, 1:100), IFNg-PerCp-Cy5.5 (4S.B3, cat.: 502526, 1:20), CD3-APC (Okt3, cat.: 17-0037-42, 1:50) (all from Biolegend, San Diego, CA, US).

Techniques: Incubation, In Vitro, Recombinant, Binding Assay, Immunopeptidomics, Sequencing, Dot Blot, Generated, Expressing, Staining, Control